Biopolym. Cell. 2004; 20(4):325-330.
Molecular and Cell Biotechnologies
Expression of extracellular human prolactin in insect cells
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Human prolactin was expressed in the insect culture cells as a soluble extracellular protein using recombinant baculoviruses. The yield varied between 20-30 mg/l medium. This recombinant prolactin was biologically active in the Nb2 rat lymphoma cells proliferation assay.
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