Biopolym. Cell. 2004; 20(4):290-294.
Structure and Function of Biopolymers
Extracellular lectins of saprophytyc bacteria Bacillus subtilis and it's mutants
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
- D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680
The influence of genotype of B. subtilis strains on the dynamics of extracellular lectins biosynthesis was studied. The spectrum of extracellular proteins presented in all strains under study contains nine main components though in different proportion. The maximum of lectin activity in the culture liquid of the wild type strain coincides with predominance of low molecular weigt proteins about 23 to 10 kDa, while in mutant strains the most intensively expressed proteins have molecular weigt of 70.4-59.0 kDa, that may be due to the aggregate formation. It was shown that mutational changes in the aminoacid pathways as well as changes in the structure of protein participating in replication and reparation resulted in strain differencies in the production and release of lectins.
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