Biopolym. Cell. 2004; 20(3):239-243.
Biomedicine
Search for optimum conditions of mobilization and conservation of the fetal liver cells of human
1Bazika D. A., 1Belyaeva N. V., 2Vasilovskaya S. V., 2Lukash L. L., 3Votyakova I. A., 2Kovalenko O. A., 2Matsuka G. Kh.
  1. Research Center for Radiation Medicine, Academy of Medical Sciences of Ukraine
    street. Melnikova, 53, Kyiv, Ukraine, 04050
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  3. Medical Center of tissue and cell therapy "Embriotech"
    5, Verkhnya Str., Kyiv, Ukraine, 01133

Abstract

Immunophenotyping the cells of embryonic liver in different conditions of immobilization (washing out the cells, trypsinization or homogenization of the tissue) has shown that the composition of cellular membrane antigens did not show significant difference. As in the case of a morphology study, the cellular samples were presented by the precursor cells, committed into the erythroid direction (50-70 %). The phenotype of those cells was CD34+/low CD117+ CD38+, HLA-DR+/low, CD90+, CD7+/low, CD45RO+/low, CD36+++, CD71+++, CD33+, CD13+ , CD14+/low CD61+/Iow. Comparative analysis has shown that the portion of a «pure» CD34+ population was the largest in the samples of cellular suspensions where the cells were isolated by trypsinization of embryonic tissue and kept in cryoconservation conditions during 3 weeks and the smallest one in the samples of cellular suspensions, obtained by homogenization of the tissue.

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