Biopolym. Cell. 1986; 2(5):251-256.
Structure and Function of Biopolymers
Analytical free flow electrophoresis of cells and proteins
- All-Union Research Institute of Applied Microbiology
Obolensk, Moscow Region, USSR
A free-flow electrophoresis device combined with optical scanner and microcomputer is described. In the device the sample is continuously pumped into the vertical stream of buffer. When constant electric field is applied at the right angle with the stream, the sample band is separated into fractions. The magnitudes of fraction displacement are measured by the scanner, and the computer provides the calculation of electrophoretic mobility of fractions. Runs last only for 10 minutes. The technique is illustrated by E. coli, sheep erythrocytes, bovine serum albumin, horse hemoglobin. It may be applied to determine isoelectric points of proteins for rapid measurement of electrophoretic mobility of cells in fermentation.
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 Abolina TA. Microelectrophoresis as a method of studying the surface structure of bacterial cells. Zh Mikrobiol Epidemiol Immunobiol. 1980;(4):21-8.
 Dukhin SS, Deryagin BV. Electrophoresis. Moscow, Nauka, 1976; 332 p.
 Troitskiy GV. Protein electrophoresis. Kharkiv, Vyshcha shkola, 1962; 319 p.
 Ramsey WS, Nowtand ED, Simpson LB. Resolution of microbial mixtures by free flow electrophoresis. Eur J Appl Microbiol Biotechnol. 1982;9(3):217-226.
 Miroshnikov IA, Fomchenkov VM, Gabuyev IS, Chekanov VA. Separation of the cell suspensions. Moscow, Nauka, 1977; 367 p.
 Hantiig K. New aspects in preparative and analytical continuous free-flow electrophoresis. Electrophoresis. 1982; 3(2):235-43.
 Silis YaYa, Kofman AM, Rozenblit AB. Initial processing of chromatograms and spectra on a computer. Principles. Algorithms. Programs. Riga, Zinatne, 1980; 128 p.
 Khemming RV. Digital filters. Moscow, Radio i sviaz, 1980; 224 p.
 Mazanov LA, Gavryushkin AV. Methods of mathematical processing of the experimental data in the free flow electrophoresis. Analysis of the distribution of the material over the cross section of the flow chamber. Molekulyarnaya biologiya, 1984; Is. 36: 34-43