Biopolym. Cell. 1986; 2(5):246-251.
Structure and Function of Biopolymers
The protein blotting
- Institute of Cytology, Academy of Sciences of the USSR
The detailed procedure of protein blotting is described in the following order: protein transfer onto the replica, blocking of the non-specific interactions, the interaction of the immobilized antigen with the 1st and 2nd antibodies, the visualization of immune complexes on the blot. Two practical applications of immunoblotting are considered when analyzing anti-oca-interferon monAbs' specificity and investigating (5-interferon expression in human HeLa cells.
 Osterman LA. The study of biological macromolecules electrofocussing, immunoelectrophoresis and radioisotope methods. Moscow, Nauka, 1983; 304 p.
 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
 O'Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975;250(10):4007-21.
 Chromatography. Electrophoresis. Immunochemistry: BioRad Catalogue K1985. Richmond : BioRad Lab., 1985. 256 p.
 Products for nucleic acids and protein research: Schleicher and Schull Catalogue. Dassel : Schleicher and Schull, 1982. 11 p.
 Aubertin AM, Tondre L, Lopez C, Obert G, Kirn A. Sodium dodecyl sulfate-mediated transfer of electrophoretically separated DNA-binding proteins. Anal Biochem. 1983;131(1):127-34.
 Reinhart MP, Malamud D. Protein transfer from isoelectric focusing Gels: the native blot. Anal Biochem. 1982;123(2):229-35.
 Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979;76(9):4350-4.
 Manabe T, Takahashi Y, Okuyama T. An electroblotting apparatus for multiple replica technique and identification of human serum proteins on micro two-dimensional gels. Anal Biochem. 1984;143(1):39-45.
 Moeremans M, Daneels G, Van Dijck A, Langanger G, De Mey J. Sensitive visualization of antigen-antibody reactions in dot and blot immune overlay assays with immunogold and immunogold/silver staining. J Immunol Methods. 1984;74(2):353-60.
 Heggeness MH, Ash JF. Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy. J Cell Biol. 1977;73(3):783-8.
 Wojtkowiak Z, Briggs RC, Hnilica LS. A sensitive method for staining proteins transferred to nitrocellulose sheets. Anal Biochem. 1983;129(2):486-9.
 Faye L, Chrispeels MJ. Characterization of N-linked oligosaccharides by affinoblotting with concanavalin A-peroxidase and treatment of the blots with glycosidases. Anal Biochem. 1985;149(1):218-24.
 Handman E, Jarvis HM. Nitrocellulose-based assays for the detection of glycolipids and other antigens: mechanism of binding to nitrocellulose. J Immunol Methods. 1985;83(1):113-23.
 Loeb MR. Immunoblot method for identifying surface components, determining their cross-reactivity, and investigating cell topology: results with Haemophilus influenzae type b. Anal Biochem. 1984;143(1):196-204.
 Grace AM, Strauss AW, Sobel BE. Sensitive quantification of isoforms of canine MM creatine kinase with an immunoblot procedure suitable for large numbers of samples. Anal Biochem. 1985;149(1):209-17.
 King SM, Otter T, Witman GB. Characterization of monoclonal antibodies against Chlamydomonas flagellar dyneins by high-resolution protein blotting. Proc Natl Acad Sci U S A. 1985;82(14):4717-21.
 Legocki RP, Verma DP. Multiple immunoreplica Technique: screening for specific proteins with a series of different antibodies using one polyacrylamide gel. Anal Biochem. 1981;111(2):385-92.
 Parekh BS, Mehta HB, West MD, Montelaro RC. Preparative elution of proteins from nitrocellulose membranes after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal Biochem. 1985;148(1):87-92.
 Anderson PJ. The recovery of nitrocellulose-bound protein. Anal Biochem. 1985;148(1):105-10.
 Papaphilis AD, Kamper EF. Assay of hybrid ribonuclease using a membrane filter-immobilized synthetic hybrid: application to the human leukemic cell. Anal Biochem. 1985;145(1):160-9.
 Lin W, Kasamatsu H. On the electrotransfer of polypeptides from gels to nitrocellulose membranes. Anal Biochem. 1983;128(2):302-11.
 Jahn R, Schiebler W, Greengard P. A quantitative dot-immunobinding assay for proteins using nitrocellulose membrane filters. Proc Natl Acad Sci U S A. 1984;81(6):1684-7.