Biopolym. Cell. 1986; 2(4):220-223.
Short Communications
Biosynthesis of different kinetic classes of nucleotide sequences in proliferating mouse plasmacytoma MOPC-21 cells
1Komissarenko S. V., 1Lukinov D. I., 1Cherepenko E. I.
  1. A. V. Palladin Institute of Biochemistry, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

MOPC-21 mouse plasmacytoma DNA was labelled in vivo during one and a half of cell-cycle period with[3H]-thymidine. This DNA was purified from cell nuclei, sonicated and fractionated on hydroxyapatite on the basis of the reassociation rate. Four kinetic classes of DNA fragments which are highly repetitive, middle repetitive, rare and unique sequences were obtained. All these sequences differed in their specific radioactivity. Highly repetitive sequences had the highest radioactivity as compared to that of the rare and unique sequences. Middle repetitive fragments had the lowest specific radioactivity. Possible reasons for the different level of radioactive thymidine incorporation are discussed.

References

[1] Edenberg HJ, Huberman JA. Eukaryotic chromosome replication. Annu Rev Genet. 1975;9:245-84.
[2] Hand R. Eucaryotic DNA: organization of the genome for replication. Cell. 1978;15(2):317-25.
[3] Calza RE, Eckhardt LA, DelGiudice T, Schildkraut CL. Changes in gene position are accompanied by a change in time of replication. Cell. 1984;36(3):689-96.
[4] Goldman MA, Holmquist GP, Gray MC, Caston LA, Nag A. Replication timing of genes and middle repetitive sequences. Science. 1984;224(4650):686-92.
[5] Naktinis VI, Maleeva NE, San'ko DF, Mirzabekov AD. Two simple methods for isolation of DNA from various sources using cetavlon. Biokhimiia. 1977;42(10):1783-90.
[6] Prima VI, Platonov OM, Gazarian KG. Reassociation of rat DNA fractions. Biokhimiia. 1980;45(3):498-506.
[7] Britten RJ, Graham DE, Neufeld BR. Analysis of repeating DNA sequences by reassociation. Methods Enzymol. 1974;29:363-418.
[8] Tarantul VZ, Gol'tsov BA, Kuznetsova ED. Structural organization of the eukaryotic genome. Molekulyarnaya biologiya. Moscow, VINITI, (Itogi nauki i tekhniki; Vol. 19). 1981; 7-83.
[9] Cherepenko EI, Lebedev VR. Characteristics of nuclear DNA of a Lepidoptera, the great wax moth Galleria mellonella, using reassociation kinetics. Mol Biol (Mosk). 1985;19(3):629-35.
[10] Bostok C, Summer AT. The eucaryotic chromosome. Amsterdam: North Holland Publ. Co., 1980. 525 p.
[11] Cech TR, Hearst JE. Organization of highly repeated sequences in mouse main-band DNA. J Mol Biol. 1976;100(3):227-56.
[12] Yamamoto M. Constitutive heterochromatin. Tanpakushitsu Kakusan Koso. 1981;26(5):694-712.