Biopolym. Cell. 1986; 2(4):217-219.
The presence of two strong promoters determines the orientation of a DNA fragment inserted into pUC19 plasmid
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
Recombinant plasmids pUC19/rpoB with and without the PJ promoter were constructed. For both plasmids stability was of 100 %. In obtained recombinant plasmids containing the PJ promoter the inserted Bglll fragment was oriented identically and in such a way that transcription from the lacUVS and PJ promoters was initiated in counter direction. Removal of the PJ promoter made the opposite orientation of the rpoB gene in recombinant plasmids possible.
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 Paton EB, Vudmaska MI, Sverdlov ED. Unidirectional orientation of the rpo B gene of E. coli cloned into filamentous M13mp8 and M13WB2348 phages. Bioorg Khim. 1984;10(11):1544-7.
 Paton EB, Woodmaska MI, Sverdlov ED. The stability of recombinant filamentous phages is reduced in the presence of the rpoB gene promoter. Biopolym. Cell. 1985; 1(3):160-2.
 Collins J. Deletions, insertions and rearrangements affecting rpoB gene expression. Mol Gen Genet. 1979;173(2):217-20.
 Zinder ND, Boeke JD. The filamentous phage (Ff) as vectors for recombinant DNA--a review. Gene. 1982;19(1):1-10.
 Maniatis T, Fritsch EF, Sambrook J. Molecular cloning - a laboratory manual. New York, Cold Spring Harbor, 1982; 545 p.
 Vel'kov VV. Instability of recombinant molecules. Genetika. 1983;19(10):1573-81.
 Gentz R, Langner A, Chang AC, Cohen SN, Bujard H. Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal. Proc Natl Acad Sci U S A. 1981;78(8):4936-40.
 Morgan BA, Kellett E, Hayward RS. The wild-type nucleotide sequence of the rpoBC-attenuator region of Escherichia coli DNA and its implications for the nature of the rifd18 mutation. Nucleic Acids Res. 1984;12(13):5465-70.