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Short Communications
The presence of two strong promoters determines the orientation of a DNA fragment inserted into pUC19 plasmid
1Paton E. B., 1Zhyvoloup A. N., 1Varanitsa L. A.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


Recombinant plasmids pUC19/rpoB with and without the PJ promoter were constructed. For both plasmids stability was of 100 %. In obtained recombinant plasmids containing the PJ promoter the inserted Bglll fragment was oriented identically and in such a way that transcription from the lacUVS and PJ promoters was initiated in counter direction. Removal of the PJ promoter made the opposite orientation of the rpoB gene in recombinant plasmids possible.


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