Biopolym. Cell. 1986; 2(4):185-189.
http://dx.doi.org/10.7124/bc.0001B2
Structure and Function of Biopolymers
Template-free ribosomal synthesis of polypeptides from aminoacyl-tRNAs : polyphenylalanine synthesis from phenylalanyl-tRNALys
1Yusupova (Tnalina) G. Zh., 2Belitsina N. V., 1Spirin A. S.
  1. Institute of Protein Research, Academy of Sciences of the USSR
    Pushchino, Moscow Region, USSR
  2. A. N. Bakh Institute of Biochemistry, Academy of Sciences of the USSR
    Moscow, USSR

Abstract

Misacylated phenylalanyl-tRNALys, similar to lysyl-tRNALys, but not phenylalanyl-tRNAPhe, is able to serve as a substrate for ribosomal synthesis of polypeptides (poly-phenylalanine and polylysine, respectively) in the absence of a template polynucleotide (poly(A)). Thus, it is the structure of tRNA that determines the ability of the amino-acyl-tRNALys to participate in the peptide elongation on ribosomes without codon-anti-codon interactions.
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