Biopolym. Cell. 1986; 2(3):136-140.
Cell Biology
Somatic hybridization of Lycopersicon esculentum Mill, and Lycopersicon peruvianum v. dentatum
1Piven N. M., 1Makhorina O. K., 1Komarnitsky I. K., 1Cherep N. N., 1Shlumukov L. R.
  1. N. G. Kholodny Institute of Botany, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


Somatic hybrids were obtained after protoplast fusion of sexually incompatible species of L. esculentum and L. peruvianum v. dentatum. Genetic novelty of the hybrids in question was shown by study of the chromosome number, isozymes (esterase, peroxidase), a small subunit of RuBPCase and chloroplast DNAs. Somatic interspecies hybrids possess new combinations of genes which have not been obtained before.


[1] Gleba YuYu, Sytnik KM. Cell engineering of plants. Kiev, Naukova Dumka, 1984; 160 p.
[2] Sidorov VA, Piven NM, Gleba YuYu, Sytnik KM. Solanaceae somatic hybridization. Kiev: Naukova Dumka. 1985; 132 p.
[3] Piven NM, Makhorina OK. Plant regeneration from callus protoplasts Lycopersicon peruvianum v. dentatum. Tsitol Genet. 1985; 19(4):271-7.
[4] Zhuchenko AA Genetics tomatoes. Kishinev: Shtiintsa, 1973. 663 p.
[5] Melchers G, Sacristan MD, Holder AA. Somatic hybrid plants of potato and tomato regenerated from protoplasts. Carlsberg Res Commun. 1978. 43(4):203-18.
[6] Melchers G. Topatoes and pomatoes somatic hybrids between tomatoes and potatoes. Tissue culture and research. Eds P. Rohlich, A. Bacsy. Budapest: Acad. Kiado, 1984:499-513.
[7] Shepard JF, Bidney D, Barsby T, Kemble R. Genetic transfer in plants through interspecific protoplast fusion. Science. 1983;219(4585):683-8.
[8] O'Connell MA, Hanson MR. Somatic hybridization between Lycopersicon esculentum and Lycopersicon pennellii. Theor Appl Genet. 1985;70(1):1-12.
[9] Menczel L, Nagy F, Kiss ZR, Maliga P. Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids. Theor Appl Genet. 1981;59(3):191-5.
[10] Gleba YuYu. Microdroplet culture. Naturwissenschaften. 1978; 65(3):158-9.
[11] Murashige T, Skoog F. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol Plant. 1962; 15(3):473-97.
[12] Cammaerts D, Jacobs M. A simple electrophoretic procedure for the determination of the polypeptide composition of the subunits of fraction 1 protein. Anal Biochem. 1980;109(2):317-20.
[13] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[14] Kolodner R, Tewari KK. Molecular size and conformation of chloroplast deoxyribonucleic acid from pea leaves. J Biol Chem. 1972;247(19):6355-64.