Biopolym. Cell. 1986; 2(3):124-129.
Structure and Function of Biopolymers
The ionic strength dependence of the cooperativity factor in DNA melting
- Institute of Molecular Genetics, Academy of Sciences of the USSR
The melting temperatures for the d(AT)24·d(AT)24 stretch located inside the DNA helix or terminally have been determined in a wide range of the ionic strength values (0.01–1 M Na+). The values of cooperativity factor were calculated from the shifts in the melting temperature of the stretch due to its different boundary conditions. With a decrease of the sodium concentration from 1 to 0.01 M the cooperativity factor has dropped by three orders of magnitude, its change being less pronounced at high ionic strengths than at low ones.
 Anshelevich VV, Vologodskii AV, Lukashin AV, Frank-Kamenetskii MD. Slow relaxational processes in the melting of linear biopolymers: a theory and its application to nucleic acids. Biopolymers. 1984;23(1):39-58.
 Amirikyan BR, Vologodskii AV, Lyubchenko YuL. Determination of DNA cooperativity factor. Nucleic Acids Res. 1981;9(20):5469-82.
 Panyutin I, Lyamichev V, Mirkin S. A structural transition in d(AT)n.d(AT)n inserts within superhelical DNA. J Biomol Struct Dyn. 1985;2(6):1221-34.
 Kozyavkin SA, Naritsin DB, Lyubchenko YuL. The kinetics of DNA helix-coil subtransitions. J Biomol Struct Dyn. 1986;3(4):689-704.
 Fixman M, Freire JJ. Theory of DNA melting curves. Biopolymers. 1977;16(12):2693-704.
 Gruenwedel DW. Salt effects on the denaturation of DNA. 3. A calorimetric investigation of the transition enthalpy of calf thymus DNA in Na2SO4 solutions of varying ionic strength. Biochim Biophys Acta. 1974;340(1):16-30.
 Gruenwedel DW. Salt effects on the denaturation of DNA. IV. A calorimetric study of the helix-coil conversion of the alternating copolymer poly[d(A-T)]. Biochim Biophys Acta. 1975;395(3):246-57.
 Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103-19.
 Gotoh O. Prediction of melting profiles and local helix stability for sequenced DNA. Adv Biophys. 1983;16:1-52.