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Cell Biology
Natural mRNA translation. I. General kinetic analysis of the process of one mRNA species translation
1Potapov A. P., 1Elskaya A. V.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


Steady-state kinetics of natural mRNA translation is theoretically analyzed. Equations are obtained for describing dependence of the total rate of cistron translation on the specific rates of each codon reading. The limited capacity of mRNA for ribosomes is shown to limit the increase of the total translation rate. Vmax depends on the sequence of mRNA codons and specific rates of codon reading. For the polyribosomal system, whose total translation rate is close to Vmax, the dependence of the total rate on specific rates of codon reading may be significantly stronger than that for the monoribosomal system. Thus, polysomal organization of the translation apparatus is suggested to be of importance for providing high susceptibility of the translation system to the regulatory factors.


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