Biopolym. Cell. 2003; 19(3):252-256.
Novel cell line with multiple markers, derivative of CHO-K1
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
A cell line CHO-K1 /pro-has been consecutively transfected with plasmids pEGFP-C1 and pTR-GT60. A subline with genotype pro– , neo+ , gfp+ , tkhv+ , hph+ has been obtained after selection in the selective media. The cells are auxotrophic mutants deficient in proline, resistant to antibiotics G-418 and hygromycin B, sensitive to Ganciclovir and produce greenish fluorescence when irradiated with long ultraviolet light.
Full text: (PDF, in Russian)
 Puck TT, Cieciura SJ, Robinson A. Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. J Exp Med. 1958;108(6):945-56.
 Kao FT, Puck TT. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc Natl Acad Sci U S A. 1968;60(4):1275-81.
 Toporova OK, Irodov DM, Sukhorada OM, Ruban TO, Kozel IuM, Andrienko BI, Novikova SM, Kordium VA. The utilization of the genes of fluorescent proteins for the visualization of the expression of genes in living cells in vitro and in vivo. The new informational technologies in medicine and ecology. X anniversary international conference and discussion scientific club: Proceedings. Yalta; Gursuf, 2002: 301-3.
 Catalogue Russian cell culture collection (RCCC). St. Petersburg; OMSK, 1999. 80 p.
 Godbey WT, Wu KK, Mikos AG. Poly(ethylenimine) and its role in gene delivery. J Control Release. 1999;60(2-3):149-60.
 Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.