Development of amperometric enzyme biosensor based on carbon fibre electrode and immobilized glucose oxidase

Danyleyko, L. V.; Schuvailo, O. N.; Arkhypova, V. M.; Soldatkin, A. P.; Dzyadevych, S. V.

doi:10.7124/bc.00063E

Biopolym. Cell. 2003; 19(1):76-80.

http://dx.doi.org/10.7124/bc.00063E

Molecular and Cell Biotechnologies

Development of amperometric enzyme biosensor based on carbon fibre electrode and immobilized glucose oxidase

1Danyleyko L. V., 1Schuvailo O. N., 1Arkhypova V. M., 1Soldatkin A. P., 1Dzyadevych S. V.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

A glucose amperometric biosensor has been developed, its characteristics being studied. The stipulation of obtaining active membrane based on glucose oxidase has been optimized. The changes in both background electrolyte concentration and buffer capacity have been shown to affect the biosensor response insufficiently. The optimum pH is found to be 7.2. The biosensor dynamic range is shown to be of 0–8 mM, the detection limit of 0.03 mM. The biosensor developed is recommended for the application in food industry to control and optimize biotechnological processes.

Full text: (PDF, in Russian)

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