Biopolym. Cell. 2002; 18(3):243-252.
http://dx.doi.org/10.7124/bc.000605
Bioorganic Chemistry
Interaction of cyanine dyes with nucleic acids. Meso-substituted trimethincyanines for fluorescent detection of nucleic acids
1Lukashov S. S., 1Losytskyy M. Yu., 2Kovtun Yu. P., 1Yarmoluk S. M.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of organic chemistry NAS of Ukraine
    5, Murmanska Str., Kyiv, Ukraine, 02660

Abstract

A series of trimethinecyanines with different meso-substituents in polymethine chain has been synthesized, and spectral luminescent properties of the dyes in the presence of double-stranded DNA, RNA and bovine serum, albumin (BSA) have been examined. The dyes fluorescence enhances up to 270-fold in the presence of nucleic acids while in the presence of protein the increase is lower. The highest rise of fluorescence intensity has been observed for the dye-nucleic acid complexes of meso-methyl-substituted dyes. When large substituents like aryl- or tret-alkyl groups are linked to the mesa-position of the dyes polymethine chain by a flexible chain from 1—3 CH^-groups, the fluorescent properties of these dyes are similar to those of the meso-methyl-substituted dyes.
Full text: (PDF, in Ukrainian)

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