Biopolym. Cell. 2002; 18(2):161-163.
Antiviral properties of de novo designed proteins based on the albeferon and the fragment LKDRHDF (30–36) from human interferon-α2
- M. M. Shemyakin and Yu. A. Ovchinnikov Institute of bioorganic chemistry, RAS
16/10, Miklukho-Maklaya Str., Moscow, Russian Federation, 117997
Two biologically active de novo proteins, ABB1-11 and ABBI-1, were engineered by including the fragment LKDRHDF (30–36) from human interferon-α2 (HuJFN-α2) into the N- and C-termini of albeferon – de novo protein with predesigned structure and function. It was shown that both proteins prevented the destruction of the L-41 and VERO cell monolayers generated by cytopathical action of a virus with the efficiency of native HuIFN-α2 and did not possess cytotoxicological properties.
 Dolgikh DA, Uversky VN, Gabrielian AE, Chemeris VV, Federov AN, Navolotskaya EV, Zav'yalov VP, Kirpichnikov MP. The de novo protein with grafted biological function: transferring of interferon blast-transforming activity to albebetin. Protein Eng. 1996;9(2):195-201.
 Fedorov AN, Dolgikh DA, Chemeris VV, Chernov BK, Finkelstein AV, Schulga AA, Alakhov YuB, Kirpichnikov MP, Ptitsyn OB. De novo design, synthesis and study of albebetin, a polypeptide with a predetermined three-dimensional structure. Probing the structure at the nanogram level. J Mol Biol. 1992;225(4):927-31.
 Zav'Yalov VP, Navolotskaya EV, Vasilenko RN, Abramov VM, Volodina EY, Roslovtseva OA, Prusakov AN, Kaurov OA. The sequence 130-137 of human interferon-alpha 2 is involved in the competition of interferon, prothymosin alpha and cholera toxin B subunit for common receptors on human fibroblasts. Mol Immunol. 1995;32(6):425-31.
 Zavyalov VP. Interferons a/b and their receptors: from structure-function studies to the design of de novo protein with the grafted active site of human interferon-a2. Proc. of the first ISTC Symp. Immunoglobulins, molecular chaperones and immunity. Turku, 1995: 45-68.
 Aphasizheva IY, Dolgikh DA, Abdullaev ZK, Uversky VN, Kirpichnikov MP, Ptitsyn OB. Can grafting of an octapeptide improve the structure of a de novo protein? FEBS Lett. 1998;425(1):101-4.
 Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: A laboratory manual. New York: Cold Spring Harbor Lab. press, 1982.
 Chizhov NP, Ershov FI, Indulen MK Fundamentals of experimental chemotherapy of viral infections. Riga: "Zinatne", 1988.