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Regulation of phosphatidylinositol 3-kinase activity during the megakaryocytic differentiation in human erythroleukemia cell line K562 induced by phorbol 12-myristate 13-acetate
- Institute of Cell Biology, NAS of Ukraine
14/16, Drahomanov Str., Lviv, Ukraine, 79005
An influence of phorbol 12-myristate 13-acetate (PMA) on the expression of p85α regulatory subunit of phosphatidylinositol (PI) 3-kinase, and on the PI 3-kinase activity during the megakaryocytic differentiation of K562 cells has been studied. It has been shown that the level of p85α expression does not change during 72 h culturing in both control and PMA-induced cells. At the same time, the coordinated changes of PI 3-kinase activity in differentiated cells in comparison with control ones suggest a differential role of PI 3-kinase signalling pathway in the proliferation regulation and in PMA-dependent megakaryocytic differentiation of K562 cells.
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