Biopolym. Cell. 2002; 18(1):62-70.
Structure and Function of Biopolymers
Investigation of aminomodified tips for atomic force microscopy of biomolecules
1, 2Limanskii A. P.
  1. Mechnikov Institute of Microbiology and Immunology NAMS of Ukraine
    14, Pushkinska Str., Kharkiv, Ukraine, 61057
  2. Arizona State University, Department of Microbiology
    Tempe, AZ 85287-2701, USA


Atomic force microscopy (AFM) was used for characterization of aminomodified tips in buffer solutions with different ionic strength (I) and pH values. Optimal conditions for tip modification in aminosilane vapour for following DNA immobilization were determined. Adhesion forces have been decreased for pair aminomodified tip — mica from I– 10 mM to I– 100 mM and pH 11.2. Functionalization of aminomodified tips by binding with glutaraldehyde and interaction with DNA was shown.


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