Biopolym. Cell. 2001; 17(6):546-550.
Molecular and Cell Biotechnologies
The expression of human alpha-2b interferon in different strains of Escherichia coli
1Slavchenko I. Yu.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The expression of human alpha-2b-interferon (IFN) in various strains of E. coli carrying the same recombinant plasmid plF-16 has been investigated. The level of IFN synthesis in different E. coli straines is shown to vary from electrophoretically undetectable amount to 35 % of the total cell proteins under the identical conditions of producer cultivation. The difference in the levels of interferon synthesis is not related to genetic instability of the plasmids.

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