Biopolym. Cell. 2001; 17(6):546-550.
Molecular and Cell Biotechnologies
The expression of human alpha-2b interferon in different strains of Escherichia coli
1Slavchenko I. Yu.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


The expression of human alpha-2b-interferon (IFN) in various strains of E. coli carrying the same recombinant plasmid plF-16 has been investigated. The level of IFN synthesis in different E. coli straines is shown to vary from electrophoretically undetectable amount to 35 % of the total cell proteins under the identical conditions of producer cultivation. The difference in the levels of interferon synthesis is not related to genetic instability of the plasmids.


[1] Hautala JA, Bassett CL, Giles NH, Kushner SR. Increased expression of a eukaryotic gene in Escherichia coli through stabilization of its messenger RNA. Proc Natl Acad Sci U S A. 1979;76(11):5774-8.
[2] Higgins CP, Smith NH. Messenger RNA processing, degradation and the control of gene expression. Regulation of gene expression-25 Years on-39 Symp. of Soc. for Gen. Microbiol. (April, 1986). Cambridge, 1986: 179-98.
[3] Pedersen S, Reeh S. Functional mRNA half lives in E. coli. Mol Gen Genet. 1978;166(3):329-36.
[4] Plautz G, Apirion D. Processing of RNA in Escherichia coli is limited in the absence of ribonuclease III, ribonuclease E and ribonuclease P. J Mol Biol. 1981;149(4):813-9.
[5] Chanda PK, Ono M, Kuwano M, Kung H. Cloning, sequence analysis, and expression of alteration of the mRNA stability gene (ams+) of Escherichia coli. J Bacteriol. 1985;161(1):446-9.
[6] Kliachko EV, Lysenko ES, Eremashvili MR, Kozlov IuI, Strongin AIa. [Mutations in Escherichia coli pmp and htpR genes stabilize the products of foreign gene expression]. Mol Biol (Mosk). 1988;22(3):760-6.
[7] Ryzhavskaia AS, Ka?dalova NV, Anukhin IuM, Kliachko EV, Livshits VA. [Stability of normal, abnormal and recombinant proteins in Escherichia coli strains deficient for intracellular proteinase La--the product of the lon gene]. Mol Biol (Mosk). 1988;22(1):201-8.
[8] Slavchenko IYu. Study bacteriophage L efficiency for human a2 interferon production in Escherichia coli. Auth. Thesis. ... kand biol nauk. Kiev, 1990. 19 p.
[9] Slavchenko IYu. Isolation of clones sensitive to bacteriophage ? from the phage resistant Escherichia coli strain. Biopolym Cell. 2001; 17(2):160-5.
[10] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
[11] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[12] AS. SSSR N 1092176. A method for preparing an artificial human interferon gene al and method for producing a polypeptide with an interferon activity microbiological synthesis. MN Kolosov, VG Korobko, VN Dobrynin, IV Severtsova, SA Chuvpilo, NS Bystrov, YuA Berlin, AL Kayushin, VV Butkus, IA Polyakova, EF Boldyreva, LS Sandakchiev, SG Popov, TN Shubina, VV Kravchenko, OI Serpinskiy, VF Yamschikov, SI Belikov, AN Sinyakov, GF Sivolobova. Publ. in BI N 18, 1984.
[13] Kravchenko VV, Guileva IP, Shamin VV, Kulichkov VA, Dobrynin VN, Filippov SA, Chuvpilo SA, Korobko VG. Construction of a tandem of artificial genes encoding human leukocyte interferon and its expression as a part of polycistronic template with coupled translation. Bioorg Khim. 1987; 13(9):1186-93
[14] Pat Ukraine N 2463. A method of producing b-galactosidase. VA Kordium, SI Chernykh, IYu Slavchenko, VG Korobko. Publ. in BI N 5-1, 1994.
[15] Slavchenko IYu, Chernykh SI, Kordium VA. The study phagedependent synthesis b-lactamase in isogenic and Su Su + E. coli strains during infection of phages Xbla and XblaQ'R'. Enzymes of microorganisms. M.: VNII-SENTI, 1989; 31-6.
[16] Litovchenko LL, Krivopalova GI, Kan'shina AV, Lebedev RL, Pustoshilova NM. The study population of recombinant strains of E. coli, synthesize TNF-alpha, TNF-beta and G-CSF, in the process of obtaining biomass. Biotekhnologiia. 2001; (3): 11-7.