Biopolym. Cell. 2001; 17(5):411-416.
Structure and Function of Biopolymers
Influence of 2', 5' oligoadenylates and theirs analogues on the cyclic nucleotides level in vivo and in vitro
1Tkachuk Z. Yu., 1Tkachuk V. V., 1Tkachuk L. V., 1Semernikova L. I., 1Matsuka G. Kh.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


It was demonstrated that 2', 5' A analogues, when introduced to organs and tissues in animals, influenced the concentration of cAMP but not cGMP. The effect depends on the concentration and, therefore, one can employ the method of testing cAMP to determine the specific action of 2', 5' A analogues, which could be used at organ transplantation and allergy.


[1] Ratner L, Wiegand RC, Farrell PJ, Sen GC, Cabrer B, Lengyel P. Interferon, double-stranded RNA and RNA degradation. Fractionation of the endonucleaseINT system into two macromolecular components; role of a small molecule in nuclease activation. Biochem Biophys Res Commun. 1978;81(3):947-54.
[2] Schmidt A, Zilberstein A, Shulman L, Federman P, Berissi H, Revel M. Interferon action: isolation of nuclease F, a translation inhibitor activated by interferon-induced (2'-5') oligo-isoadenylate. FEBS Lett. 1978;95(2):257-64.
[3] Ratner L, Sen GC, Brown GE, Lebleu B, Kawakita M, Cabrer B, Slattery E, Lengyel P. Interferon, double-stranded RNA and RNA degradation. Characteristics of an endonuclease activity. Eur J Biochem. 1977;79(2):565-77.
[4] Vilcek S. Inducia enzymovych aktivit ? bunkach po posobeni interferonu. Chem Listy. 1984; 78:400-32.
[5] Itkes AV, Tunitskaia VL, Severin ES. [Mechanisms of the regulation of the biological activity of the cell by 2',5-oligoadenylate]. Biokhimiia. 1985;50(4):531-42.
[6] Pat. USA 5,571,799. (2'—5') Oligoadenylate analogues useful as inhibitors of host-V5-graft response. Z. Tkachuk, E. Kvasyuk, G. Matsuka, I. Mikhailopulo. Publ. Nov. 5, 1996.
[7] Itkes AV. Oligoadenylate and cyclic AMP: interrelation and mutual regulation. Prog Mol Subcell Biol. 1994;14:209-21.
[8] Mikhailopulo IA, Baran EA, Tkachuk LV. Synthesis and use in kidney transplantations in rabbits and monkey of (2'—5')-oligoadenylate trimers modified at the 2', 5'-terminus. 4th Int. Symp Mol Aspects of Chemother. (23—25 June, 1993, Gdansk). Gdansk, 1993; 61.
[9] Turakulov YaKh, Satov TS, Khalikov SK, Isaev EI, Gaynutdinov MKh. Cyclic nucleotides and regulation of cellular metabolism. Tashkent: Fan, 1983; 240 p.
[10] Lambert M, Christophe J. Stimulation of adenosine triphosphatase (ATPase) by calcium, magnesium, and bicarbonate in rat pancreatic plasma membranes [proceedings]. Arch Int Physiol Biochim. 1976;84(5):1084-5.
[11] Tkachuk ZYu, Rybalko SL, Semernikova LI, Tkachuk VV, Zavelevich M. P., Tkachuk LV, Mikhailopulo IO, Matsuka GKh. The effect of trimeric 2',5'-oligoadenylic acid and its epoxyderivative on the human immunodeficiency virus reproduction and retroviruses reverse transcriptase activity. Biopolym Cell. 1999; 15(4):314-8.