Biopolym. Cell. 2001; 17(2):140-146.
Structure and Function of Biopolymers
DNA hypomethylation of sunflower seedlings
1Tishcnenko E. N., 1Korzh L. P.
  1. Institute of Plant Physiology and Genetics, NAS of Ukraine
    31/17, Vasylkivska, Kyiv, Ukraine, 03022


The DNA methylation in shoots and roots of etiolated seedlings of sunflower (Helianthus annuus L.) has been investigated. Using the methyl-sensitive enzymes of restriction we showed that CpCpGpG-, CpCpA/TpGpG- and GpApApTpTpC- sites are subjected to differential methylation. In particular, these DNA motifs are extensively hypomethylated in shoots, where in contrast to roots, diverse genes are expressed almost 4 times more. The organogenesis in the sunflower callus culture of the shoot apical meristem is also accompanied by the reduction in the level of cytosine enzymatic modification in CpCpGpG- and CpCpA/TpGpG-seguences of DNA. A rote of the DNA enzymatic methylation variability during growth and differentiation of sunflower is under discussion.


[1] Finnegan EJ, Genger RK, Peacock WJ, Dennis ES. DNA METHYLATION IN PLANTS. Annu Rev Plant Physiol Plant Mol Biol. 1998;49:223-47.
[2] Wang L, Heinlein M, Kunze R. Methylation pattern of Activator transposase binding sites in maize endosperm. Plant Cell. 1996;8(4):747-58.
[3] Meyer P, Niedenhof I, ten Lohuis M. Evidence for cytosine methylation of non-symmetrical sequences in transgenic Petunia hybrida. EMBO J. 1994;13(9):2084-8.
[4] Singal R, Ferris R, Little JA, Wang SZ, Ginder GD. Methylation of the minimal promoter of an embryonic globin gene silences transcription in primary erythroid cells. Proc Natl Acad Sci U S A. 1997;94(25):13724-9.
[5] Bianchi MW, Viotti A. DNA methylation and tissue-specific transcription of the storage protein genes of maize. Plant Mol Biol. 1988;11(2):203-14.
[6] Riggs CD, Chrispeels MJ. The expression of phytohemagglutinin genes in Phaseolus vulgaris is associated with organ-specific DNA methylation patterns. Plant Mol Biol. 1990;14(4):629-32.
[7] Matzke MA, Matzke A. How and Why Do Plants Inactivate Homologous (Trans)genes? Plant Physiol. 1995;107(3):679-685.
[8] Walbot V, Warren C. DNA methylation in the Alcohol dehydrogenase-1 gene of maize. Plant Mol Biol. 1990;15(1):121-5.
[9] Brusslan JA, Karlin-Neumann GA, Huang L, Tobin EM. An Arabidopsis mutant with a reduced level of cab140 RNA is a result of cosuppression. Plant Cell. 1993;5(6):667-77.
[10] Kakutani T, Jeddeloh JA, Flowers SK, Munakata K, Richards EJ. Developmental abnormalities and epimutations associated with DNA hypomethylation mutations. Proc Natl Acad Sci U S A. 1996;93(22):12406-11.
[11] Li E, Bestor TH, Jaenisch R. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell. 1992;69(6):915-26.
[12] Finnegan EJ, Peacock WJ, Dennis ES. Reduced DNA methylation in Arabidopsis thaliana results in abnormal plant development. Proc Natl Acad Sci U S A. 1996;93(16):8449-54.
[13] Sheldon CC, Burn JE, Perez PP, Metzger J, Edwards JA, Peacock WJ, Dennis ES. The FLF MADS box gene: a repressor of flowering in Arabidopsis regulated by vernalization and methylation. Plant Cell. 1999;11(3):445-58.
[14] Tishchenko EN, Kuntsevich VI, Bilinskaya AT, Lobov VP. Nuclear RNA in Helianthus annuus vegetative organs. Fiziologiia rasteniy. 1996; 43(2):213-9.
[15] Tishchenko EN. Differential methylation of external cytosine residues of CCGG seguences in DNA of sunflower seedlings. Dopovidi Nats Akad Nauk Ukrainy. 1999; (10):165-7.
[16] Palmgren G, Mattsson O, Okkels FT. Specific Levels of DNA Methylation in Various Tissues, Cell Lines, and Cell Types of Daucus carota. Plant Physiol. 1991;95(1):174-8.
[17] Kunakh VA. Plant genome variation in the course of in vitro dedifferentiation and callus formation. Fiziologiia rasteniy. 1999; 46(6):919-29.
[18] Loschiavo F, Pitto L, Giuliano G, Torti G, Nuti-Ronchi V, Marazziti D, Vergara R, Orselli S, Terzi M. DNA methylation of embryogenic carrot cell cultures and its variations as caused by mutation, differentiation, hormones and hypomethylating drugs. Theor Appl Genet. 1989;77(3):325-31.
[19] Morrish FM, Vasil IK. DNA Methylation and Embryogenic Competence in Leaves and Callus of Napiergrass (Pennisetum purpureum Schum.). Plant Physiol. 1989;90(1):37-40.