Biopolym. Cell. 2000; 16(3):236-239.
Methods
Proteins and cyanine dyes. 1. Asymmetrical carbocyanine dye 14K – a new fluorescent probe for homogeneous detection of proteins in solution
1Yarmoluk S. M.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

An interaction of carbocyanine 14K (6,8-diethyl-7-[E-3-(8-ethyl-7,8-dihydro-[l,3]-thiazolo-[4',5',3,4], benzo-(d]-[1,2,3]-thiadiazolo-7-iliden)-l-propenyl]-6H-imidazolo-[4',5', 5, 6]tiodiazolo-8-ium iodide) with four proteins in both native and denatured states has been studied. The difference in fluorescent enhancement of this dye in the presence of various proteins has been shown. The fluorescence intensity of 14K in the presence of denatured proteins increased by 25–40 % in comparison with the fluorescence intensity of this dye in the complexes with corresponding native proteins. A possible mechanism of increasing the dye fluorescence under the interaction with proteins and nucleic acids has been proposed. The influence of different substances on fluorescent properties of 14K has been studied.

References

[1] Scopes R. K. Protein purification: principles and practice. 2nd ed. Amsterdam: Springer, 1987:280—283.
[2] Korneliuk AI, Kurochkin IV, Matsuka GKh. [Tyrosyl-tRNA synthetase from the bovine liver. Isolation and physico-chemical properties]. Mol Biol (Mosk). 1988;22(1):176-86.
[3] Haughland R. P. Detection and quantitation of protein in solution. Handbook of fluorescent probes and research chemicals. 6th ed. 1996:180-2.
[4] Mayer A, Neuenhofer S. Luminescent Labels - more than just an alternative to Radioisotopes? Angew Chem Int Ed Engl. 1994;33(10):1044–72.
[5] Yarmoluk SM. Interaction of cyanine dyes with nucleic acids. 8. Investigation of cyanine dyes as fluorescent probes for the nucleic acids detection. Biopolym Cell. 1999; 15(4):328-36.
[6] Kovalenko EA. Amonoacid and monosaccharide content of Bacillus genus bacteria extracellular sialospecific lectins. Mikrobiol Z. 1997; 59(5):3-6.
[7] Privalov PL. Study of proteins in the denatured state: Proc. All-Union. Symposium. protein chemistry. Tbilisi
[8] Demchenko AP. UV spectrophotometry and structure of proteins. Kiev: Naukova Dumka, 1981.
[9] Ishchenko AA. Structure and spectral-luminescent properties of polymethyne cyanine dyes. Kyiv: Naukova dumka 1994. 232 p.