Biopolym. Cell. 1999; 15(6):501-509.
Cell Biology
Shikonin accumulation and cytological peculiarities of highly productive Arnebia euchroma cell strain upon surface and submerged maintenance
1Poronnyk O. A., 1Kunakh V. A., 1Adonin V. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


A. euchroma novel cell strain AE-3, accumulating about 80 and 100 mg/1 shikonin per day upon maintenance in suspension culture and on agarized medium, respectively, has been studied. The study on cell and nucleus size, their morphology, evaluation of mitotic activity, chromosome number, level and types of their aberration failed to demonstrate any essential difference under the various conditions of maintenance. The strain appears to be mixoploid showing chromosome variation range from 10 to 130 chromosomes with modal classes formed by cells carrying 30 to 37 chromosomes and characterized by low level of antiphase chromosome aberration (about 6 %). Comparison of various A. euchroma cell lines has not revealed any relationship between their productivity and cytological and cytogenetic peculiarities.


[1] Tabata M., Fujita Y. Production of shikonin by plant cell cultures. Biotechnology In Plant Sci. Relevance to Agriculture In Eighties, New York: Acad press 1985 pp. 207-218.
[2] Fukui H., Tsukada M., Mizukami H., Tabata M. Formation of stereoisomeric mixtures of naphthoquinone derivatives in Echium lycopsis callus cultures. Phytochemistry, 1983; 22(2):453-456.
[3] Zhu R., Cao R., Wang M., Pan IX, Du Z., Lu W., Shi Y. Dark red pigment produced in calluses Onosma paniculatum. Acta Botanica Sinica. 1990. 32(10):315—320.
[4] Shcherbanovskiy L. P., Shubina L. S, Shcherbanovskaya F. R. Naphthoquinone higher plants. Rastitel'nyye resursy. 1971; 7(4):606—615.
[5] Al K., Li F., Li Y., Wang W., Wu Y. Study naphthoquinone components Onosma confertum and quantification of shikonin. Acta Botanica Sinica. 1989; 31(7):549—553.
[6] Zhou L., Zheng G., Wang S. Callus culture and education shikonin Onosma paniculatum. Acta bot. Yun nanica. 1991; 13(3): 749—753.
[7] Kunakh VA, Poronnyk OO, Zakhlenjuk OV, Adonin VI. New shikonin producing cell lines of Arnebia euchroma (Royle) Jonst. Obtaining and characteristics. Fiziologiia i biokhimiia kul'turnykh rasteniy. 1999; 31(3):208-13
[8] Davydenkov V. P., Patudin A. V., Popov K. G., Rabinovich S. A., Miroshnikoye A. I. Cell Culture Arnebia euchroma (Royle) Jonst - new source of shikonin. Khim. farm. zhurn. 1991. 1: 53-55.
[9] Kunakh VA, Levenko BA. Modification of the squash preparation method for plant tissue culture cells. Tsitol. Genet. 1975 9(1):56–58.
[10] Chromosome numbers of flowering races genius. Ed. AA Fedorov. Leningrad: Nauka, 1969. 926.
[11] Chromosome numbers of flowering plants of the USSR flora. Ed. AL Takhtadzhian. Leningrad: Nauka, 1990. 508p
[12] Kunakh V. A. Features of structural mutagenesis populations cultured plant cells. Advances sovrem genetics. Nauka, Moscow, 1984. Issue 12: 30-62.
[13] Kunakh V. A. Genome variability and accumulation of indoline alkaloids in Rauwolfia serpentina Benth. Cell culture. Biopolym. Cell. 1994; 10(1):3-30
[14] Kunakh V. A. Somaclonal variation in Rauwolfia. Biotechnol. Agr. and Forest. 1996. 36: 315—332.
[15] Zagoskina NV, Fedoseeva VG, Frolova LV, Azarenkova ND, Zaprometov MN. Tissue culture of tea plant: differentiation, ploidy level, and formation of phenolic compounds. Fiziologiia rasteniy. 1994. 41(5): 762-7.