Biopolym. Cell. 1999; 15(3):220-226.
http://dx.doi.org/10.7124/bc.00051C
Structure and Function of Biopolymers
The different factors influence on the serum immunoglobulms transformation into polyreactive immunoglobulins and their interaction with antigens
1Bobrovnik S. A., 1Chornaya V. E., 1Nikolaenko I. V.
  1. Palladin Institute of Biochemistry, NAS of Ukraine
    9, Leontovycha Str., Kyiv, Ukraine, 01601

Abstract

The optimal conditions were studied for the process of serum immunoglobulins activity increasing using sharp shift of pH or their incubation with chaotropic ions. It was shown that this process strongly depends on the temperature of incubation. The influence of pH and concentration of some salts on binding of polyreactive immunoglobulins (PRIG) to plate immobilised antigens were studied. The data obtained show that electrostatic forces play an important role in the PRIG-Ag interaction. It was also shown, that no: only serum immunoglobulins, but also highspecific monoclonal antibodies could be transformed into PRIG using high or low pH, or ctaoiropic ions.
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