Biopolym. Cell. 1998; 14(6):544-548.
Cell Biology
The influence of granulocyte colony-stimulating factor «Granocyte» on active oxygen forms generation by neutrophils in vitro
1Kovalenko E. I., 1Semenkova G. N., 2Smirnova E. N., 1Cherenkevich S. N., 1Razumovich Y. A., 3Gerein V.
  1. Belarusian State University
    4, Fr. Scorina Minsk ave., Belarus, 220050
  2. Belarusian Research Center for Pediatric Oncology, Hematology and Immunology under the Ministry of Healthcare of Republic of Belarus
    43, Frunzenskaya Str., v. Borovlyani, Minsk region, 223053
  3. Children Onco-Hematological Clinic
    Gummersbach, Germany

Abstract

Granulocyte colony-stimulating factors arc used at the therapy of myelosupressive conditions. The influence of granulocyte colony-stimulating factor «Granocyte» in vitro on active oxygen forms generation by neutrophils of healthy donors and myeloperoxidase activity of these cells was investigated at the adhesion of cells on tlif glass surface and the action of latex, phyfohemagglutinin and arachidonic acid. Researches were curried out by chemiluminescenl method with use of luminol. It was shown, that «Granocyte» increases the yield of active oxygen forms in neutrophils of healthy donors in vitro at the adhesion of cells on the glass. «Granocyte» induces the clianges of the kinetics of active forms generation at actions of cell metabolism stimulators for different healthy donors. Myeloperoxidase activity of neutrophils is not changed under the influence of granulocyte colony-stimulating factor «Granocyte» in vitro. It was supposed, that granulocyte colony-stimulating factor «Granocyte» modifies activity of the plasma membranes redox-systems, but does not influence on intracellular oxidases.

References

[1] Taylor KM, Jagannath S, Spitzer G, Spinolo JA, Tucker SL, Fogel B, Cabanillas FF, Hagemeister FB, Souza LM. Recombinant human granulocyte colony-stimulating factor hastens granulocyte recovery after high-dose chemotherapy and autologous bone marrow transplantation in Hodgkin's disease. J Clin Oncol. 1989;7(12):1791-9.
[2] Nissen C, Carbonare V, Moser Y. In Vitro Comparison of the Biological Potency of Glycosylated versus Nonglycosylated rG-CSF. Drug Invest. 1994;7(6):346–52.
[3] Semenkova GN, Smirnova E. , Kovalenko EI et al Chemiluminescent monitoring of neutrophils functional activity in oncohematological patients at the exit from neutropenia suite: Abstracts of 2nd ICCC (April 27-30). Berlin. 1996. A12.
[4] Smirnova EN, Semenkova GN, Kovalenko EI et al. The generation of active oxygen forms and granulocytopoiesis in patients with acute lymphoblastic leukemia at therapy with use granulocyte colonystimulating factor. Ann Hematol. 1997; 74 (Suppl. 1):A30.
[5] Semenkova GN, Smirnova EN, Kovalenko EI, Gerin V, Savanovich II, Cherenkevich SN Oxygen-activating ability of neutrophils from children with non-Hodgkin's lymphomas in the therapy with G-CSF. Experimental Oncology. 1997; 19(2): 153-6.
[6] Boyum A. Isolation lymphocytes, granulocytes and macrophages. Lymphocytes: isolation, fractionation and characterisation. Univ Scan J Immunol. 1976; 5: 9-15.
[7] Vetoshin SS, Semenkova GLL, Cherenkevich SN. Chemiluminescent methods for analysis of cell activation. Luminescent analysis in biomedical research. Riga, 1987: 81-5.
[8] Semenkova GN, Cherenkevich SN, Levin VI, Svirnovski? AI. [Generation of active forms of oxygen by human peripheral blood neutrophils and lymphocytes during adhesion to glass]. Biofizika. 1985;30(5):864-7. Russian.
[9] Allen RC, Stjernholm RL, Steele RH. Evidence for the generation of an electronic excitation state(s) in human polymorphonuclear leukocytes and its participation in bactericidal activity. Biochem Biophys Res Commun. 1972;47(4):679-84.
[10] Ohsaka A, Saionji K, Sato N, Mori T, Ishimoto K, Inamatsu T. Granulocyte colony-stimulating factor down-regulates the surface expression of the human leucocyte adhesion molecule-1 on human neutrophils in vitro and in vivo. Br J Haematol. 1993;84(4):574-80.
[11] Barsukov AA, Filatov AV, VAsin YuA et al. Isolation of oxygen active forms in the macrophages adhesion. Immunologiia. 1983; 1: 69-73.
[12] Semenkova GN, KOvalenko EI, Smirnova EI et al. Effect of granulocyte-colony stimulating factor G CSF on recovery granulocytopoiesis and functional activity of neutrophils in hematologic malignancies. Proc. rep. 2nd Congress of Belarusians Soc Photobiologists and Biophysics "Molecular Cell Biology Sciences». Minsk, 1996: 176.
[13] Nauseef WM. Myeloperoxidase deficiency. Hematol Pathol. 1990;4(4):165-78.