Biopolym. Cell. 1998; 14(3):203-209.
Structure and Function of Biopolymers
Structural and functional studies of cardiac tropomyosin as; autoantigen at dilated cardiomyopathy
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Tropomyosin have been purified to homogeneity from normal, and affected by dilated cardiomyopathy (DCMP) human myocardium and from skeletal muscle as well. The molecular masses of both preparations of cardiac tropomyosins were identical. The immuno-screening of DCMP patients sera by EUSA method have shown the presence of autoantibodies directed against cardiac tropomyosin in the limited number of sera. No immunoreactivity have been detected against skeletal tropomyosin. Western blot analysis detected any linear determinants in tropomyosin molecule under denaturating conditions. We suggest that cardiac tropomyosin doesn't undergoes sufficient structural changes at dilated cardiomyopathy progression.
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