Biopolym. Cell. 1998; 14(1):39-42.
Structure and Function of Biopolymers
Effect of 80S ribosomes on higher eukaryotic leucyl-tRNA synthetase activity at the first stage of tRNA aminoacylation
1Belyanskaya L. L., 1Turkovskaya H. V., 1Kovalenko M. I., 1El'skaya A. V.
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
80S ribosomes stimulate activity of homologous leucyl-tRNA synthetase on the first stage of tRNA aminoacylation, namely for formation enzyme bound aminoacyl-adenylate. A possible mechanism and functional significance of the interaction between eukaryotic leucyl-tRNA synthetase and ribosomes are discussed.
Full text: (PDF, in Russian)
References
[1]
Kisselev LL, Favorova OO, Lavrik OI. Biosynthesis of proteins from amino acids to aminoacyl-tRNA. Moscow, Nauka, 1984; 408 p.
[2]
Ivanov LL, Kovalenko MI, Turkovskaia GV, El'skaia AV. [Structure-functional properties of eukaryotic aminoacyl-tRNA synthetase]. Biokhimiia. 1992;57(8):1123-41.
[3]
Mirande M. Aminoacyl-tRNA synthetase family from prokaryotes and eukaryotes: structural domains and their implications. Prog Nucleic Acid Res Mol Biol. 1991;40:95-142.
[4]
Kisselev LL, Wolfson AD. Aminoacyl-tRNA synthetases from higher eukaryotes. Prog Nucleic Acid Res Mol Biol. 1994;48:83-142.
[5]
Ryazanov AG, Ovchinnikov LP, Spirin AS. Development of structural organization of protein-synthesizing machinery from prokaryotes to eukaryotes. Biosystems. 1987;20(3):275-88.
[6]
Sana Sara, Mozuraitis RJ, Kharchenko OV, Ivanov LL, Turkovskaya GV, Martinkus ZP, Kovalenko MI, El'skaya AV. Interaction of eukaryotic aminoacyl-tRNA synthetases with ribosomes. Biopolym Cell. 1992; 8(1):101-7.
[7]
Sana Sara, Ivanov LL, Turkovskaya GV, Martinkus ZP, Kovalenko MI, El'skaya AV. Effect of ribosomes on the thermostability of rabbit liver aminoacyl-tRNA synthetases. Biopolym Cell. 1992; 8(3):6-9.
[8]
Potapov AP, Ovcharenko GV, Soldatkin KA. Isolation and characterization of 40S-and 60S-ribosomal subunits from rabbit liver. Methods mol biol. Naukova dumka, 1986: 100-5.
[9]
Lemoine F, Waller JP, van Rapenbusch R. Studies on methionyl transfer RNA synthetase. 1. Purification and some properties of methionyl transfer RNA synthetase from Escherichia coli K-12. Eur J Biochem. 1968;4(2):213-21.
[10]
Ovcharenko GV, Ivanov LL. Methods for determining the enzymatic activity of aminoacyl-tRNA synthetases. Methods of molecular biology. Kiev: Naukova dumka, 1979: 133-9.
[11]
Ogata K, Kurahashi A, Kenmochi N, Terao K. Role of 5SrRNA as a positive effector of some aminoacyl-tRNA synthetases in macromolecular complexes, with specific reference to methionyl-tRNA synthetase. J Biochem. 1991;110(6):1037-44.
[12]
Reed VS, Wastney ME, Yang DC. Mechanisms of the transfer of aminoacyl-tRNA from aminoacyl-tRNA synthetase to the elongation factor 1 alpha. J Biol Chem. 1994;269(52):32932-6.
