Structure rearrangement of plasmid pATV8 in transgenic mice. I. Study of structure of the integrated transgenome and biological effects of transgenosis

Chashchina, L. I.; Petrenko, A. I.; Vagina, I. N.; Krysan, K. V.; Solomko, A. P.

doi:10.7124/bc.000492

Biopolym. Cell. 1997; 13(4):323-327.

http://dx.doi.org/10.7124/bc.000492

Molecular Mechanisms of Differentiation

Structure rearrangement of plasmid pATV8 in transgenic mice. I. Study of structure of the integrated transgenome and biological effects of transgenosis

1Chashchina L. I., 1Petrenko A. I., 1Vagina I. N., 1Krysan K. V., 1Solomko A. P.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Interactions of avion Rons sarcoma proviral DNA with mouse genome during transgenesis lead to significant modifications of the vector molecule. Two stable genetic structures derived from transgenome were discovered: extrachromosomal circle DNA and DNA, integrated into mouse genome. In the integrated DNA there was a complete loss of pwviral DNA sequences and a partial deletion of bacterial vector plasmid. The. presence of exogenic DNA in transgenic mice cells had a profound influence on the function of their genome, characterized by the increase in tumorigenity and the decrease of fertility. Genetical and physiological changes induced by the transgenome are the consequence of its interactions with the mice genome. Our results indicate that the transgene caused the structural and functional destabilization of mouse genome.

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