Biopolym. Cell. 1997; 13(3):218-221.
Genome and Its Regulation
Physical mapping of Malacosoma neustria nuclear polyhedrosis virus genome
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
A physical map of the circular M. neustria nuclear polyhedrosis virus genome was constructed. The complete order of BamHI, KpnI and PutI restriction enzyme sites was determined. The polyhedrin gene was localized on the map. The size of the viral DNA was calculated to be about 139 kbp.
 Granados RR, Federici BA. The biology of baculoviruses. Practical application for insect control. Boca Raton: CRC press, 1986, Vol. 2:
 Miller LK. Baculoviruses as gene expression vectors. Annu Rev Microbiol. 1988;42:177-99.
 Maeda S. Gene transfer vectors of a baculovirus. Bombyx mory nuclear polyhedrosis virus, and their use for expression of foreign genes in insect cells. Invertebrate Cell Systems and Applications. Ed. J. Mitsuhashi. Boca Raton: CRC press, 1984. Vol. I: 167-81.
 Skuratovskaya IN, Strokovskaya LI, Komissarenko SV, Mendeleva NV. Structure and functioning of the nuclear polyhedrosis virus genome in Melacosoma neustria. Tsitol Genet. 1986; 20(1):31-6.
 Strokovskaya LI, Veselovsky OV, Kikhno IM, Mirjuta NYu. Cloning of the Malakosoma neustria nuclear polyhedrosis virus polyhedrin gene. Biopolym Cell. 1990; 6(3):81-4.
 Petrenko OI, Strokovskaya LI, Solomko AP. Nucleotide sequence of MnNPV polyhedrin gene. Biopolym Cell. 1994;10(3-4):105.
 Strokovskaya LI, Kikhno IM, Veselovsky OV, Skuratovskaya IN, Miryuta NYu, Petrenko AI, Solomko AP. Malacosoma neustria nuclear polyhedrosis virus expression vector. Biopolym Cell. 1990; 6(3):84-9.
 Sukhorada EM, Kanyuka VYu, Miloserdova VD. Reproduction of nuclear polyhedrosis silkworm virus with prolonged passaging in cells transplanted lines insects. Entomopathogens. viruses and Scient. value. Kiev: Naukova Dumka. 1981: 44-50.
 Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975;98(3):503-17.
 Cherepenko EI, Martynenko EI. A simple method for baculovirus DNA isolation. Mol Biol (Mosk). 1985; 19(8): 1519-24.
 Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a Laboratory Manual. New York: Cold Spring Harbor Lab., 1982. 452 p.
 Vlak JM, Smith GE. Orientation of the Genome of Autographa californica Nuclear Polyhedrosis Virus: a Proposal. J Virol. 1982;41(3):1118-21.