Biopolym. Cell. 1996; 12(4):88-93.
Phycobiliprotein pigments for biomolecules. 1. Preparative fractionation and spectral characteristics
1Chernenko V. Y., 1Kiryushyn A. V., 1Lukash L. L.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


The authors have elaborated technological approaches concerning preparative fractionation of phycobiliproteins – phycoerythrin from a microalga Porphyridium cruentum, and of phycocyanin from a cyanophyte alge Anabaena variabilis. The spectral properties of dyes obtained have been also investigated.


[1] Oi VT, Glazer AN, Stryer L. Fluorescent phycobiliprotein conjugates for analyses of cells and molecules. J Cell Biol. 1982;93(3):981-6.
[2] Cordier G. Flow cytometry for immunology. Biol Cell. 1986;58(2):147-50.
[3] Antibodies, A practical approach. Ed Catty D. 1989 IRL Press Oxford. 259p
[4] Andreyuk EI, Kopteva ZhP, Zanina VV Cyanobacteria. K.: Naukova Dumka, 1990. 197 p.
[5] AS. N 1400275 (USSR). Separation method a1-antitrypsin in the fraction. GG Demidov, V. Yu Chernenko, KA Odynets, LE Korol.
[6] Troitskiy GV, Azhitskiy BYu. Isoelectric focusing of proteins in self-organizing and artificial pH gradients. Kiev: Naukova Dumka, 1984. 215 p.
[7] Paster EU, Ovod VV, Pozur VK, Vikhot' NE. Immunologiya. Prakticum. K. : Visha shk., 1989; 128-30.
[8] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[9] Patent USA N 4 859 582 Fluorescent conjugates for analysis of molecules and cells. L. Stryer, A. Glazer, V. Oi. 1989.