Biopolym. Cell. 1996; 12(1):64-68.
Mapping of Bacillus subtilis riboflavin operon fragment coding for 3,4-dihydroxy-2-butanone-4-phosphate synthase
1Boretska N. I., 1Liauta-Teglivets O. Ye., 1Voronovsky A. Y., 1Boretsky J. R., 1Shavlovsky G. M.
  1. Division of Cell Regulatory Systems of O.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine
    14/16, Drahomanov Str., Lviv, Ukraine, 79005

Abstract

Complementation analysis of Bacillus subtilis riboflavin operon was carried out by means of Escherichia coli riboflavin deficient strains and recombinant plasmids. It was found that ORF-3 ofB. subtilis riboflavin operon encodes for a bifunctional enzyme. It catalyses formation of both 2, 5-diamino-4-hydroxy-6-ribosylamino-pyrimidine-5'-phosphate from GTP and 3, 4-dihydroxy-2-butanone-4-phosphate from ribuhse-5-phosphate.

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