Biopolym. Cell. 1994; 10(5):98-104.
The DNA probe construction with use the silver salt. 2. The colouring of the monomer and the single-stranded DNA
1Lichina M. V., 1Shugalii A. V.
  1. Institute of Chemical Physics, RAS in Chernogolovka
    Academician Semenov avenue 1, Chernogolovka, Moscow region, Russian Federation, 142432

Abstract

Some aspects of adenine modification as a one of DNA base during the preparing of coloured metal-complex is under investigation. After the N-metoxyadenine formation as a result of formaldehyde reaction with the amino/imino groups of the base the lengthing of lateral chain is occured at the expense of ethanolamine and some metoxy groups. The colour of modified adenine is developed after a silvre addition and the ultimate branched metal-complex possess in addition the fluorescent properties. The kind of clour is determined by pH of incubation media, the violet probe with a 10–15 % modification level is obtained in an alkaline solution in the course of 15–20 minutes.

References

[1] Lichina MV, Shugalii AV. The DNA probe construction with use the silver salt. 1. The formation and characterization of the oligomer coloured chains. Biopolym Cell. 1994; 10(2):38-44. ttp://
[2] Chichibabin LE. Basic Principles of Organic Chemistry. M.: GHI, 1963; Vol 1. 910 p.
[3] Eyring EJ, Ofengand J. Reaction of formaldehyde with heteroocyclic imino nitrogen of purine and pyrimidine nucleosides. Biochemistry. 1967;6(8):2500-6.
[4] McGhee JD, von Hippel PH. Formaldehyde as a probe of DNA structure. II. Reaction with endocyclic imino groups of DNA bases. Biochemistry. 1975;14(6):1297-303.
[5] Kochetkov NK, Budovskiy EI, Sverdlov ED. et al. Organic chemistry of nucleic acids. M.: Chemistry, 1970. 720 p.
[6] Hoeltke HJ, Ettl I, Finken M, West S, Kunz W. Multiple nucleic acid labeling and rainbow detection. Anal Biochem. 1992;207(1):24-31.