Biopolym. Cell. 1994; 10(3-4):93-99.
Cloning of human ARSs
1Fedorenko E. S., 1Irodov D. M., 1Lihacheva L. I., 1Podolskaya S. M., 1Kordium V. A.
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
In this study we cloned two fragments of total DNA of human genome from leukocytes and placental cells, which capable provide of autonomously state of pYF40 cloning vector (in which there was consisted) in Sacch. cerevisiae. The obtained fragments have in l ength: 6,5 (from leukocyte) and 3,4 kb (from placental cells), respectively. This fragments can provide high transformation in yeast (102–103) transformants (10 ng plasmid DNA). We have obtained long expression of apo-Al human gene carried by extrachromosomal recombinant DNA, which contain cloned ARSs (autonomously replications sequences).
Full text: (PDF, in Russian)
References
[1]
Duesberg PH. Transforming genes of retroviruses. Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:13-29. Review.
[2]
Sorge J, Wright D, Erdman VD, Cutting AE. Amphotropic retrovirus vector system for human cell gene transfer. Mol Cell Biol. 1984;4(9):1730-7.
[3]
Myers RM, Tjian R. Construction and analysis of simian virus 40 origins defective in tumor antigen binding and DNA replication. Proc Natl Acad Sci U S A. 1980;77(11):6491-5.
[4]
Hanahan D, Lane D, Lipsich L, Wigler M, Botchan M. Characteristics of an SV40-plasmid recombinant and its movement into and out of the genome of a murine cell. Cell. 1980;21(1):127-39.
[6]
O'Hare K, Benoist C, Breathnach R. Transformation of mouse fibroblasts to methotrexate resistance by a recombinant plasmid expressing a prokaryotic dihydrofolate reductase. Proc Natl Acad Sci U S A. 1981;78(3):1527-31.
[7]
Mellon P, Parker V, Gluzman Y, Maniatis T. Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system. Cell. 1981;27(2 Pt 1):279-88.
[8]
Mitrani-Rosenbaum S, Maroteaux L, Mory Y, Revel M, Howley PM. Inducible expression of the human interferon beta 1 gene linked to a bovine papilloma virus DNA vector and maintained extrachromosomally in mouse cells. Mol Cell Biol. 1983;3(2):233-40.
[9]
Di Maio D, Maniatis T. Intact bovine papilloma virus-human DNA recombinant plasmides that propagate as episomes is mouse cells and bacteria. Eukaryotic viral vectors. Ed . Y. Gluzman. New York: Cold Spring Harbor. Lab., 1982: 937.
[10]
Oganesian NA, Chepurnoĭ AI, Vel'kov VV, Kopylova-Sviridova TN, Fodor II. Study of the stability of hybrid plasmids replicating in Saccharomyces cerevisiae due to DNA fragments from polyoma virus. Mol Biol (Mosk). 1984;18(1):21-9.
[11]
Montiel JF, Norbury CJ, Tuite MF, Dobson MJ, Mills JS, Kingsman AJ, Kingsman SM. Characterization of human chromosomal DNA sequences which replicate autonomously in Saccharomyces cerevisiae. Nucleic Acids Res. 1984;12(2):1049-68.
[12]
Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
[13]
DNA Cloning: a practical approach. Ed. Glover DM. Vol. III. Oxford University Press. 1987; 272 p
[14]
Adams RLP. Cell Culture for Biochemists. Elsevier. North-Holland Biomedical Press. 1980; 292 p.
[15]
Shulzhenko VN, Kordyum VA. Cloning of the Zea maize genome DNA sequences capable of autonomous replication in Saccharomyces cerevisiae. Biopolym Cell. 1987; 3(5):270-4.
[16]
Klimov AN, Usatenko MS, Denisenko AD, Kozhevnikova KA, Oleĩnik IA. Isolation of apolipoproteins A-I, A-II and E and their estimation by rocket immunoelectrophoresis in blood plasma of dis-alpha-lipoproteinemic patients. Biokhimiia. 1981;46(4):590-602.
[17]
Levy HB, Sober HA. A simple chromatographic method for preparation of gamma globulin. Proc Soc Exp Biol Med. 1960;103:250-2.
[18]
Wilson WB, Nakane PK. Immunofluorescence and related staining techniques. Eds W. Knapp et al. Amsterdam, 1978: 215-24.
[19]
Jayaram M, Li YY, Broach JR. The yeast plasmid 2mu circle encodes components required for its high copy propagation. Cell. 1983;34(1):95-104.
