Biopolym. Cell. 1994; 10(3-4):89-92.
Creation of the expressing constructions with bar gene for plant transformation
1Zayas A. I., 1Stekhin I. N., 1Putilina N. V., 1Levenko B. A.
  1. Institute of Plant Physiology and Genetics, NAS of Ukraine
    31/17, Vasylkivska, Kyiv, Ukraine, 03022


Phosphinothrioin (PPT) is a potent inhibitor of glutamine synthetase in bacteria and plants and is used as a non-selective herbicide. The bar gene which confers resistance ire Streptomyces hygroscopicus to bialaphos, a tripeptide containing PPT, was cloned. The bar gene was placed under control of the 35S promoter of the cauliflower mosaic virus for transferring to plant cells using agrobacterium–mediated transformation.


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