Biopolym. Cell. 1994; 10(3-4):84-88.
Cloning of the herbicide bialaphos resistance gene
1Zayas A. I., 1Stekhin I. N., 1Putilina N. V., 1Levenko B. A.
  1. Institute of Plant Physiology and Genetics, NAS of Ukraine
    31/17, Vasylkivska, Kyiv, Ukraine, 03022

Abstract

A gene which confers resistance to the herbicide bialaphos (bar) has been cloned from Streptomyces hygroscopicus genomic DNA. The bar gene was cloned in E. coli using the vector pUCfff. The recombinant clones, resistant to bialaphos in concentration 0,005 mg/ml, were obtained. Plasmid DNA isolated from resistant clones in each case contained the same 1,7-kb PstI fragment. Restriction mapping of this fragment has been made.

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