Biopolym. Cell. 1994; 10(3-4):79-83.
Genetic mechanisms of Escherichia coli cell resistance to amino acid antimetabolites. 1. Search for genes other than glyphosate target gene
1Cherepenko E. I., 1Karpenko O. I., 1Maluta S. S.
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Abstract
Conjugation donor Hfr C prototrophic cells were treated with NNG and glyphosate resistant cells were selected. Out of 50 mutants obtained 36 transferred the trait of glyphosate resistance to recipient cells showing that only a single gene was mutated. No changes in glyphosate penitration into mutant cells studied were shown. No changes in ColiE of mutant DNA were found which could be if pronounced amplification of aroA locus occurred. Mutants obtained were shown to be localized at 4 located far from each other and from aroA gene.
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