Biopolym. Cell. 1994; 10(3-4):75-78.
ColEl plasmids can prevent thermoinactivation of phenylalanyl-tRNA synthetase in Escherichia coli
1Cherepenko E. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Because RNA I controlling ColEl plasmid replication is tRNA-like structure and has consensus and homological sequences of tRNAphe we studied the ability of this molecule to complement thermolabile phenylalanyl-tRNA synthetase in vivo just like tRNAphe can do. We showed that cells containing this enzyme and transformed with pUC19 and pBR322 plasmids could grow at 42 °C when grown overnight with aeration in diluted meat broth incubating all transformants at a once which resulted from transformation procedure being diluted by a factor of 50.


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