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ColEl plasmids can prevent thermoinactivation of phenylalanyl-tRNA synthetase in Escherichia coli
- Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
Because RNA I controlling ColEl plasmid replication is tRNA-like structure and has consensus and homological sequences of tRNAphe we studied the ability of this molecule to complement thermolabile phenylalanyl-tRNA synthetase in vivo just like tRNAphe can do. We showed that cells containing this enzyme and transformed with pUC19 and pBR322 plasmids could grow at 42 °C when grown overnight with aeration in diluted meat broth incubating all transformants at a once which resulted from transformation procedure being diluted by a factor of 50.
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