Biopolym. Cell. 1994; 10(2):52-56.
http://dx.doi.org/10.7124/bc.0003A4
Detection of biological activity of human interleukin 2 which obtained by microbiological synthesis in E. coli directed by c-DNA gene
1Glushakova L. G., 2Kovalchyuk E. N., 1Prima V. I., 1Kordyum V. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
    45, Vasilkivska Str., Kyiv, Ukraine, 01022

Abstract

The constructions with direct and inverse orientations of c-DNA interleukin 2 gene has been obtained by the use of commercial vector system (pET) created by W. Studier et al. for E. coli . The detection of biological activity of interleukin 2 produced by microbiological synthesis in E. coli has been carried out.
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