Biopolym. Cell. 1994; 10(1):76-81.
Template specificity and exonuclease activites of DNA-polymerases of cyanobacteria plectonema boryanum
- D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680
Functional characteristics of DNA-dependent DNA-polymerases of cyanobacteria P. boryanum have been studied. DNA-polymerase II (DP-II) have been determined to use activelly the templates with various structures (the most active was the enzyme on DNA P. boryanum and cyanophage LPP-3 which lyse this cyanobacteria); DNA-polymerase I (DP-I) was less active on the most templates tested excluding cyanobacterial DNA. Both enzymes need priming of the synthetic ribonucleic templates and they manifested differ abilities to include each of desoxynucleosidtriphosphates. DP-I and DP-II have 3'–5' exonuclease activity, moreover, DP-II has more potent associated exonuclease. Only DP-I is capable of hydrolysing phosphodiether bonds in directions of 5'– 3'. The conclusion has been made of possible functional role of cyanobacterial DNA-polymerases in vivo: DP-II is the principal cellular replicative enzyme, and DP-I is responsible for realization of reparative processes and close in properties to DNA-polymerase I from E. coli.
 Lysenko TG, Shainskaya OA, Gerashchenko IV, Syrchin SA. Comparison of two methods for obtaining cell-free extracts of cyanobacteria for studying the activity of dehydrogenases. Mikrobiol Zh. 1989; 51(3):87-91.
 Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
 Mendzhul MI, Nesterova NV, Sukhanov SN. Extraction and partial purification of DNA-dependent DNA-polymerase from cells of cyanobacterium Plectonema boryanum. Mikrobiol Zh. 1993; 55(2):46-51.
 Mendzhul MI, Syrchin SA, Averkiev AA, Busakhina IV. Comparison of different methods of cyanophage LPP-3 isolation and its DNA. Mikrobiol Zh. 1992; 54(2):70-4.
 Mendzhul MI, Syrchin SA, Averkiev AA, Rebentish BA. The way of defence of cyanophage LPP-3 DNA against restriction-modification systems in the cells cyanobacterium Plectonema boryanum. Biopolym Cell. 1993; 9(5):54-61.
 Methods of molecular genetics and genetic engineering. Ed. RI Salganik. Novosibirsk: Nauka, 1990: 28 p.
 Sukhanov SN, Nesterova NV, Mendzhul MI. Physicochemical properties and sensitivity to inhibitors of cyanobacterium Plectonema boryanym DNA-polymerases. Mikrobiol Zh. 1993; 55(5):42-50.
 Bollum FJ. Filter paper disk techniques for assaying radioactive macromolecules. Methods Enzymol. 1968;169вЂ“73.
 H?bscher U. DNA polymerases in prokaryotes and eukaryotes: mode of action and biological implications. Experientia. 1983;39(1):1-25.
 Klenow H, Henningsen I. Selective elimination of the exonuclease activity of the deoxyribonucleic acid polymerase from Escherichia coli B by limited proteolysis. Proc Natl Acad Sci U S A. 1970;65(1):168-75.
 Holler E, Fischer H, Simek H. Non-disruptive detection of DNA polymerases in nondenaturing polyacrylamide gels. Eur J Biochem. 1985;151(2):311-7.
 Nossal NG. Prokaryotic DNA replication systems. Annu Rev Biochem. 1983;52:581-615.
 Porter RD. DNA transformation. Methods Enzymol. 1988;167:703-12.