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Analysis of transgenic plants using polymerase chain reaction: the pair of universal primers for amplification of 35S promoter sequence
- Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680
The pair of primers for amplification of the 35S CaMV promoter sequence using polymerase chain reaction have been designed. The primers allow to carry out screening of transgenic plants transformed with any construction containing this promoter. A possibility to screen transgenic plants of several species including N. tabacum, P. satlvum, T. vulgaram, B. oleracea, B. napus has been showed.
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