Biopolym. Cell. 1985; 1(4):213-218.
Biopolymers and Genome Regulation
Steroid-receptor complexes and mechanism for regulation of the eucaryotic genome transcription
- K. A. Timiryazev Institute of Plant Physiology, Academy of Sciences of the USSR
The in vitro and in vivo interaction of steroid-receptor complexes with nucleic acids and chromatin is studied. The highly purified glucocorticoid-receptor complex is shown to possess a pronounced selectivity of its association with nucleic acids depending on their base composition and sequence. It is stated that the rat liver glucocorticoid receptors bind to definite sites of homologous DNA with an increased affinity; these sites are involved into the of transcriptionaly active chromatin under the effect of in vivo glucocorticoids on living cells. Studies of DNA methylation level in rat liver and its chromatin sensitivity to DNAase I during hormonal induction, have permitted concluding that hormone-responsive chromatin loci possess a distinct В«potentially active conformation. Thus, hormonal responsiveness of genes depends on DNA sequence as well as on the way of their organization within chromatin. Intracellular RNA may compete with DNA for receptor binding and thereby moderate the intensity of hormonal influence on chromatin.
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 Kuz'menko AP, Dashkevich VS, Romanov GA, Vaniushin BF, Salganik RI. Transition of the specific binding sites of glucocorticoid receptor complexes from the fraction of potentially active DNA into the fraction of transcriptionally active DNA under cortisol induction in rat liver cells. Dokl Akad Nauk SSSR. 1983;272(3):739-41.
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 Romanov GA, Gorskova VA. Purification and comparative study of the properties of glucocorticoid- and estrogen-receptor complexes in the rat liver. Biokhimiia. 1984;49(1):3-11.
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 Romanov GA, Zhavoronkova EN, Savel'ev SV, Vaniushin BF. Nuclease sensitivity and methylation of chromatin DNA of the rat liver in the initial period of glucocorticoid activity. Probl Endokrinol (Mosk). 1984;30(6):38-42.
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 Romanov GA, Vanyushin BF. Cytosol induces apparent selectivity of glucocorticoid receptor binding to nucleic acids of different secondary structure. Biochim Biophys Acta. 1982;699(1):53-9.
 Romanov GA. Possible function of RNA-binding proteins of eukaryotes: blocking of RNA accessibility for DNA-interacting (regulatory) protein. Biokhimiia. 1982;47(3):339-42.