Biopolym. Cell. 1985; 1(3):160-162.
Short Communications
The stability of recombinant filamentous phages is reduced in the presence of the rpoB gene promoter
1Paton E. B., 1Woodmaska M. I., 2Sverdlov E. D.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR
  2. M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR
    Moscow, USSR


A fragment of DNA including the rpoB gene coding for the p-subunit of rifampicin-resistant RNA-polymerase of E. coli and rplL and rplJ genes encoding ribosomal protein synthesis as well as two promoters Pj and PP was cloned into the filamentous M13mp9 phage. The stability of the recombinant phages was shown to increase as the result of the elimination of the strong Pj promoter.


[1] Paton E. B., Woodmaska M. I., Sverdlov E. D. Unidirectional orientation of rpoB gene of E. coli cloned into filamentous phages M13mp8 and M13WB234. Russian Journal of Bioorganic Chemistry. 1984; 10 (11):1544-7.
[2] Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning: a laboratory manual New York: Cold Spring Harbor Lab. publ., 1982 545 p.
[3] Barnes WM, Bevan M. Kilo-sequencing: an ordered strategy for rapid DNA sequence data acquisition. Nucleic Acids Res. 1983;11(2):349-68.
[4] Messing J. An integrative strategy of DNA sequencing and experiments beyond. In: Recombinant DNA: Third Cleveland Symp. on macromolecules Ed. A. Walton. Amsterdam: Elsevier, 1981, p. 143-153.
[5] The molecular biology catalogue. Uppsala: Pharmacia P-L biochemicals, 1983. 43 p.
[6] Morgan BA, Kellett E, Hayward RS. The wild-type nucleotide sequence of the rpoBC-attenuator region of Escherichia coli DNA and its implications for the nature of the rifd18 mutation. Nucleic Acids Res. 1984;12(13):5465-70.
[7] Newman AJ, Ma JC, Howe KM, Garner I, Hayward RS. Evidence that rifampicin can stimulate readthrough of transcriptional terminators in Escherichia coli, including the attenuator of the rpoBC operon. cleic Acids Res. 1982;10(22):7409-24.
[8] Collins J. Deletions, insertions and rearrangements affecting rpoB gene expression. Mol Gen Genet. 1979;173(2):217-20.
[9] Remaut E, Stanssens P, Fiers W. Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda. Gene. 1981;15(1):81-93.