Biopolym. Cell. 1985; 1(3):156-158.
Short Communications
A study of lysine operon organization in Bacillus subtilis
1Aleksieva Z. M., 1Shevchenko T. N., 1Malyuta S. S.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


The pBR322 plasmid was used for constructing pLBl plasmid carrying genes of Bacillus subtills lysine biosynthesis. B. subtilis and E. coli strains (lysine-auxotrophs) are transformed. The plasmid is shown to complement both mutations in seven genes of lysine biosynthesis in E. coli cells and four mutations in B. subtills. The size of DNA insertion, according to the elctrophoresis data, is 4.5×106.


[1] Umbarger HE. Amino acid biosynthesis and its regulation. Annu Rev Biochem. 1978;47:532-606.
[2] Bachmann BJ, Low KB. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980;44(1):1-56.
[3] Okunev OV, Shevchenko TN, Maliuta SS. Cloning of the Bacillus subtilis DNA fragment containing the genes for lysine and riboflavin biosynthesis. Genetika. 1984;20(7):1061-6.
[4] Shevchenko TN, Okunev OV, Aleksieva ZM, Maliuta SS. Expression of the genes for lysine biosynthesis of Bacillus subtilis in Escherichia coli cells. Tsitol Genet. 1984; Jan-Feb;18(1):58-60.
[5] Dauce-Le Reverend B, Boitel M, Deschamps AM, Lebeault J-M, Sano K, Takinami K, Patte J-C. Improvement of Escherichia coli strains overproducing lysine using recombinant DNA techniques. Eur. J. Appl. Microbiol., 1982; 15(4):227-231.