Biopolym. Cell. 1985; 1(1):26-32.
Cell Biology
Protein composition and organization of the nuclear matrix
- N. K. Koltsov Institute of Developmental Biology, Academy of Sciences of the USSR
Moscow, USSR
Abstract
In proliferating cells the nuclear matrix differs in higher content of lamin B and lower content of polypeptides in the region of about 30 kilodaltons. In hepatomas and other tumours in addition to these features the content of high molecular weight (higher than 100 kilodalton) polypeptides is considerably elevated in the nuclear matrix as well as in the residual protein fraction. Granules, 25-30 nm in diameter, similar to annular granules of the pore complexes are observed in the nuclear matrix. These granules may be isolated after treatment with dilute alkali or hyaluronidase. High molecular weight polypeptides of the alkali-insoluble fraction of the nuclear matrix, as well as lamins, are revealed by immunofluorescence at the periphery of the interphase nucleus, however, during mitosis they are spread all over the cell. The proteins of the nuclear matrix intensely incorporate the labelled precursors, the incorporation into the most high molecular weight and into the most lower molecular weight polypeptides being inhibited by chloramphenicol.
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