Biopolym. Cell. 1985; 1(1):21-25.
Biopolymers and Genome Regulation
Selectivity of mutagenic action of synthetic polynucleotides
1Alexandrov Yu. N., 1Gershenson S. M.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


Solutions of high molecular weight synthetic polyriboadenylic, polyriboinosylic, polyribouridylic, polyriboadenylic-cytidylic, polydeoxyadenylic and -polydeoxythymidylic acids injected into Drosophila melanogaster males caused a 6-16 time increase of the overall frequency of recessive lethals in the 2nd chromosome. Complementation analysis of the induced lethals showed an extremely high locus-specificity for some of these mutagens. Distribution along the chromosome of induced lethals is different for different polynucleotides. Possible mechanisms of the mutagenic action of synthetic polynucleotides are discussed.


[1] Gershenson SM, Alexandrov YuN, Malyuta SS. Mutagenic Effect of DNA and viruses in Drosophila. Kiev: Naukova Dumka, 1975; 160 p.
[2] Fahmy OG, Fahmy MJ. Differential induction of chromosome deletions by natural and synthetic macromolecules in Drosophila melanogaster. Genetics. 1965;52(5):861-73.
[3] Alexandrov YuN, Gershenzon SM. Mutagenic effect of two synthetic polyribonucleotides and its high specificity. Mol Biol., 1982. 30:16—19.
[4] Ives PT. The Genetic Structure of American Populations of Drosophila Melanogaster. Genetics. 1945;30(2):167-96.
[5] Wallace B. Allelism of second chromosome lethals in D. melanogaster. Proc Natl Acad Sci U S A. 1950;36(11):654-7.
[6] Simmons MJ, Crow JF. Mutations affecting fitness in Drosophila populations. Annu Rev Genet. 1977;11:49-78.
[7] Abrahamson S, Wurgler FE, DeJongh C, Meyer HU. How many loci on the X-chromosome of Drosophila melanogaster can mutate to recessive lethals? Environ Mutagen. 1980;2(4):447-53.
[8] Rodin SN. Analysis of allelic relationships among recessive lethals induced in Drosophila by exogenous DNA and by some viruses. Genetika. 1974; 10(9):94-105.
[9] Alexandrov YuN, Golubovsky D. The multisite mutations induced by viruses and foreing DNA can spread in natural populations of Drosophila. Drosophila Inform. Serv., 1983; 59(1):10—11.
[10] Alexandrov YuN, Gershenzon SM. The specificity of the mutagenic action of DNA of different origin. In the book.: Molecular mechanisms of genetic processes: Proc. of reports. III Proc. symposium. M., 1976, p. 14-15.
[11] Khesin RB. Genome instability. Moscow, Nauka, 1984; 472 p.
[12] Khesin RB, Leybovich BA. The structure of chromosomes, histones and the activity of genes in Drosophila. Molekulyarnaya Biologiya, 1976, 10(1):3—33.