Biopolymers and cell. 2010; 26 (4): 299 - 305
Ischemic stroke in Ukrainian population: possible involvement of the F2 G20210A, F5 G1691A and MTHFR C677T gene variants
P. F. Tatarskyy1,3, A. M. Kucherenko1, 3, S. A. Kravchenko1, D. V. Shulzenko2, S. M. Kuznetsova2, L. A. Livshits1
1Institute of molecular biology and genetics NAS of Ukraine 150, Zabolotnogo Str, Kyiv Ukraine, 03680
2 Gerontology Institute MAS of Ukraine, 67, Vyshgorodska Str., Kyiv, Ukraine, 04114
3 National Taras Shevchenko University of Kyiv, 64, Volodymyrska Str, Kyiv, Ukraine, 01033
Aim. To evaluate a possible involvement of the F2, F5, MTHFR gene variants into ischemic stroke pathogenesis in population of Ukraine. Methods. Polymorphic variants were analyzed in unrelated 183 stroke patients, 100 individuals from the general population of Ukraine and 88 healthy individuals elder than 65 years using PCR followed by RFLP analysis. Results. Unfavourable polymorphic variants F2 20210A, F5 1691A and MTHFR 677T were observed more frequently in patients with ischemic stroke comparing to control groups. Conclusions. F5 1691A and MTHFR 677T polymorphic variants are associated with the occurrence of ischemic stroke in women. F2 20210A is associated with the occurrence of ischemic stroke in men. Cumulative risk factor for stroke development is revealed in a combination of unfavorable polymorphic variants 20210A, 1691A and 677T of F2, F5 and MTHFR genes.
Keywords: Factor V Leiden (F5 G1691A) gene, prothrombin (F2 G20210A) gene, methylenetetrahydrofolate reductase (MTHFR C677T) gene, ischemic stroke, combined genotype.