Biopolymers and cell. 1990. Volume 6. № 1. 100 - 103
L. G. Glushakova, O. R. Romanovskaya, V. A. Kordlum
COMPARISON OF EFFICIENCY OF USE OF EARLY AND LATE PROMOTERS OF r/Phage FOR 0-GALACTOSIDASE GENIE EXPRESSION IN ESCHERJCH1A COLI
Summary
The model system for comparison of the efficiency of use of early and late promoters of T7 phage for the [5-galactosidase gene expression has been constructed. The following strategy of construction has been chosen. The gene of T7 phage RNA-polymerase has been inserted into DNA of phage cloning vector. The model gene z of lac operon of E. coli (EcoRI-Sall digest of pLZ56) has been inserted into DNA of plasmid GEM-1 (firm «!Promega») which contains phage T7 late promoter. The cloned pLZ56 fragment of DNA contains also S-D sites and A3 promoter specifically recognized by E. coli RNA-polymerase. Some features of b-galactosidase synthesis during transcrip¬tion of the z gene under the control of the late promoter of phage T7 and under the control of A3 promoter are shown.