Isolation and restriction analysis of two plasmids from Streptomyces globisporus 1912

Authors

  • B. P. Matselyukh D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine 154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680 Author
  • V. V. Lukyanchuk D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine 154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680 Author
  • L. V. Polishchuk D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine 154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680 Author
  • A. B. Matselyukh D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine 154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680 Author
  • J. Rohr Georg-August-Universitat Gottingen, Institute for Organic and Biomolecular Chemistry Tammannstr. 2, 37077 Gottingen, Germany Author

DOI:

https://doi.org/10.7124/bc.0004D3

Abstract

Two plasmids pSG1912-1 (10,3 kb) and pSG1912-2 (22,4 kb) were isolated from mycelium of three Streptomyces globisporus strains differing in the level of sporulation and landomycin E production. The number of restriction sites for endonucleases Bglll, Kpnl, PviJI and Sad was determined in both plasmid molecules.

References

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Published

1998-05-20

Issue

Section

Gene-Engineering Biotechnology