Modified methods of cultivation and fixation of human amniocytes
DOI:
https://doi.org/10.7124/bc.0004FBAbstract
Modifications offered for classic techniques of cultivation and fixation of amniocytes have been described. The modified technique allows to shorten the time for cultivation till 8–12 days, increase a number of metaphase plates on the analysed preparations and to improve their quality.References
Prospects of Medical Genetics. Ed. NG Bochkov. M.: Meditsina, 1982. 500 p.
Kuleshov YaP, Bartseva OV, Baronov VS et al. Prenatal cytogenetic diagnosis: results of the studies I and II trimesters of pregnancy. Medical genetics: Theory and Practice (Results of cooperation of the Institute of med. Genetics AMS USSR and the Institute of Obstetrics and Gynecology AMN SSSR). M., 1989: 83-91.
* Tabor Ann. Genetic amniocentesis-indication and risk. '. Prenat. Diagn. 1988. 35, N 6: 520- 537.
Mennuti MT. Prenatal diagnosis--advances bring new challenges. N Engl J Med. 1989;320(10):661-3.
Wolstenholme J, Rooney DE, Davison EV. Confined placental mosaicism, IUGR, and adverse pregnancy outcome: a controlled retrospective U.K. collaborative survey. Prenat Diagn. 1994;14(5):345-61.
Jacobson CB, Barter RH. Intrauterine diagnosis and management of genetic defects. Am J Obstet Gynecol. 1967;99(6):796-807.
Nadler HL. Indications for amniocentesis in the early prenatal detection of genetic disorders. Birth Defects Orig Artic Ser. 1971;7(5):5-9.
Nelson MM, Emery AE. Amniotic fluid cell cultures. J Med Genet. 1973;10(1):19-22.
