Biopolym. Cell. 1992; 8(3):53-56.
Short Communications
The effect of neomycin on poly(U) and poly(dT) translation in cell-free systems from Saccharomyces cerevisiae and Neurospora crassa
1Shul'ga N. I., 1Potapov A. P.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The effect of different concentration of neomycin on poly(U) and poly(dT) translation has been studied in cell-free systems from S. cerevisiae and M. crassa. The data show that this translation is carried out by cytoplasmic ribosomes. Mg2+ iones stimulate poly(U) and poly(dT) translation in these systems and neomycin unlike the prokaryotic cell-free systems inhibits translation of both templates. It was proposed that ribosome decoding center was changing during evolution to higher stereospecificity to the codon-anticodon sugar-phosphate backbone structure.

References

[1] Potapov AP. A stereospecific mechanism for the aminoacyl-tRNA selection at the ribosome. FEBS Lett. 1982;146(1):5-8.
[2] Potapov AP. Mechanism of the stereospecific stabilization of codon-anticodon complexes in ribosomes during translation. Zh Obshch Biol. 1985;46(1):63-77.
[3] Potapov AP, Soldatkin KA, Soldatkin AP, El'skaya AV. The role of a template sugar-phosphate backbone in the ribosomal decoding mechanism. Comparative study of poly(U) and poly(dT) template activity. J Mol Biol. 1988;203(4):885-93.
[4] Groisman IS, Potapov AP. The effect of Escherichia coli ribosomal protein S12 mutations on the efficiency of deoxyribonucleotide matrix poly(dT) translation. Biopolym Cell. 1989; 5(6):101-4.
[5] Potapov AP, Soldatkin KA, Soldatkin AP, El'skaya AV. Comparative study of poly(U) and poly(dT) template activity in cell-free protein synthesizing systems from Escherichia coli and wheat germ. Biopolym. Cell. 1988; 4(3):133-8.
[6] Tuite MF, Plesset J. mRNA-dependent yeast cell-free translation systems: theory and practice. Yeast. 1986;2(1):35-52.