Biopolym. Cell. 2021; 37(5):335-345.
Структура и функции биополимеров
Характеристика регенерированного кератина и пленки на его основе
1Гавриляк В. В., 2Михалюк В. В., 1Кочубей В. В.
  1. Национальный университет «Львовская политехника»
    12, ул Степана Бандеры, Украина, 79013
  2. Институт биологии животных НААН
    ул. Стуса, 38, Львов, Украина, 79034

Abstract

Цель. Исследовать характеристики регенерированного кератина, полученного из волоса человека методом сульфитолиза, создать на его основе пленки и изучить их поверхности, а также адсорбционной способности. Методы. Кератины экстрагировали сульфитолизом. Структуру регенерированного кератина исследовали методом ИК-спектроскопии, термогравиметрии и электрофореза. Для формирования пленок раствор кератина выливали на чашки Петры. Их биосовместимость анализировали в адсорбционном тесте путем инкубации в сыворотке крови человека. Для оценки морфологии поверхности пленок использовали сканирующую электронную микроскопию. Результаты. Регенерированный кератин представлен преимущественно протеинами интермедиальных филаментов и характеризуется лучшими термическими свойствами, чем исходный. Пленки на основе кератина имели ярко выраженную рельефную структуру. В адсорбционном тесте показано преимущественную способность пленок адсорбировать на своей поверхности альбумин из сыворотки крови человека. Выводы. Мягкая экстракция кератинов методом сульфитолиза обеспечивает сохранение их природных свойств и способность пленок на основе кератина формировать связи с протеинами крови.
Keywords: биополимеры, кератин, сульфитолиз, термогравиметрический анализ, пленки, адсорбция

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